Table 2

Organ clearance of ¹²⁵I-labeled cationized and native humanized 4D5 after intravenous injection in the rat

4D5	Parameter	Organ
4D5	Parameter	Brain	Heart	Lung	Liver	Kidney
Cationized 4D5	V_d(μl/g)	27 ± 3	414 ± 17	4391 ± 928	11354 ± 1848	1030 ± 118
Native 4D5	V_d (μl/g)	13 ± 1	91 ± 3	242 ± 65	230 ± 102	81 ± 7
Cationized 4D5	PS (μl/min/g)	0.087 ± 0.019	0.34 ± 0.10	22.1 ± 5.6	59 ± 8	4.7 ± 0.7
Native 4D5	PS (μl/min/g)	<0.02	0	0	0.75 ± 0.69	0
Cationized 4D5	%ID/g	<0.02	0.039 ± 0.018	2.5 ± 1.1	6.0 ± 0.5	0.52 ± 0.16
Native 4D5	%ID/g	<0.02	0	0	0.72 ± 0.57	0

Data are expressed as mean ± S.E. of three rats. Measurements were 120 min after injection. The V_o values used for calculation of PS products are: brain (10 μl/g), heart (150 μl/g), lung (225 μl/g), liver (119 μl/g) and kidney (131 μl/g), as reported previously (Pardridge et al, 1994a).