Effects of treatments on MDCK cell number and size
| Cell number | Cell size | |||
|---|---|---|---|---|
| Control | Treated | Control | Treated | |
| Cells/175-cm2 flask × 10−6 | μg of protein/106 cells | |||
| Phenylephrine (100 μM, n = 30) | 36 ± 3 | 37 ± 2 | 155 ± 7 | 156 ± 8 |
| PMA (100 nM, n = 21) | 39 ± 3 | 30 ± 2c | 160 ± 9 | 172 ± 9 |
| H7 (100 μM, n = 4) | 57 ± 10 | 19 ± 3b | 130 ± 16 | 163 ± 13 |
| Staurosporine (100 nM, n = 4) | 44 ± 4 | 12 ± 2b | 133 ± 14 | 433 ± 63a |
| KT5926 (1 μM, n = 4) | 28 ± 1 | 11 ± 1c | 208 ± 4 | 356 ± 15b |
| W-7 (30 μM, n = 4) | 37 ± 4 | 34 ± 3 | 186 ± 12 | 186 ± 8 |
Effects of the indicated concentrations of pharmacological agents were tested during a 24-hr treatment of the cells in 10% serum-containing medium compared with cells treated with the respective vehicle in parallel flasks.
abc P < .05, .01 and .0001, respectively, in a paired, two-tailed t test.