Cell preparation | % Loss in Tryptase Activity | |
---|---|---|
Without heparin | With heparin | |
Tonsil | 78 ± 8.51-151 | 24 ± 8.51-150 |
Skin | 85 ± 5.41-151 | 23 ± 1.41-150 |
Medium alone | 92 ± 2.6 | 38 ± 2.01-150 |
Tryptase was added in the presence or absence of 30 μg/ml heparin, and BAPNA cleaving activity was determined in supernatants after centrifugation at 4°C for 10 min. Values are mean ± S.E. for three separate experiments.