BrEA | DHEA | |||||
---|---|---|---|---|---|---|
Inhibitor concentration (μM) | 25 | 50 | 25 | 50 | 100 | 250 |
% Inhibition of G6PDH | 49 ± 0 | 71 ± 8 | 77 ± 3 | 82 ± 3 | 93 ± 3 | — |
Assays of G6PDH in airway smooth muscle cell lysates were performed on 100 μl of cell lysate, to which inhibitors were added. Activity is expressed as % inhibition of DMSO vehicle-treated controls by 16α-BrEA (BrEA) or DHEA). DMSO alone caused no reduction in G6PDH activity under assay conditions. Each value represents the mean of at least three experiments. Methods are described in the text.