Table 6

Total extracellular and cellular Hg content in renal DT cells incubated with 5 μM Hg: Effect of extracellular thiols

Incubation/time	Extracellular Hg	Cellular Hg
min	pmol
Experiment 1
Buffer/1	2605 ± 817	904 ± 276
Buffer/5	2121 ± 551	1046 ± 212
Buffer/10	1831 ± 355	1505 ± 269
Buffer/60	1603 ± 242	1609 ± 359
BSA/1	3858 ± 134	121 ± 12^6-a
BSA/5	3509 ± 404^6-a	110 ± 16^6-a
BSA/10	3618 ± 349^6-a	119 ± 18^6-a
BSA/60	3568 ± 252^6-a	158 ± 22^6-a
Experiment 2
Buffer/1	1723 ± 402	616 ± 114
Buffer/5	1254 ± 225	2109 ± 518
Buffer/10	1233 ± 118	2287 ± 586
Buffer/60	870 ± 77	2335 ± 605
DMPS/1	3780 ± 470^6-a	84.0 ± 28.9^6-a
DMPS/5	4343 ± 393^6-a	108 ± 23^6-a
DMPS/10	3723 ± 695^6-a	86.3 ± 28.1^6-a
DMPS/60	4327 ± 548^6-a	111 ± 28^6-a
Experiment 3
Buffer/1	3512 ± 595	633 ± 90
Buffer/5	2910 ± 242	1269 ± 404
Buffer/10	2378 ± 336	1996 ± 238
Buffer/60	2061 ± 164	2256 ± 244
GSH/1	4150 ± 99	185 ± 25^6-a
GSH/5	4038 ± 181^6-a	185 ± 26^6-a
GSH/10	3863 ± 350^6-a	204 ± 24^6-a
GSH/60	3326 ± 453^6-a	341 ± 44^6-a

Hg accumulation in renal DT cells was measured as described in the legend to table 1 except that final Hg and thiol concentrations were 5 and 20 μM, respectively. Results are the mean ± S.E. of measurements from four or five separate cell preparations.

↵6-a Significantly different (P < .05) from corresponding value in paired samples incubated with Hg alone (“buffer”).