Table 5

Total extracellular and cellular Hg content in renal PT cells incubated with 5 μM Hg: Effect of extracellular thiols

Incubation/time	Extracellular Hg	Cellular Hg
min	pmol
Experiment 1
Buffer/1	2565 ± 611	666 ± 138
Buffer/5	2121 ± 465	1174 ± 198
Buffer/10	1930 ± 413	1312 ± 238
Buffer/60	1658 ± 337	1454 ± 276
BSA/1	3616 ± 313	92.8 ± 18.5^5-a
BSA/5	3494 ± 308^5-a	139 ± 13^5-a
BSA/10	3392 ± 472^5-a	162 ± 21^5-a
BSA/60	3682 ± 236^5-a	185 ± 25^5-a
Experiment 2
Buffer/1	2505 ± 648	822 ± 301
Buffer/5	988 ± 142	1328 ± 80
Buffer/10	1131 ± 333	1529 ± 151
Buffer/60	1110 ± 275	1427 ± 78
DMPS/1	5056 ± 195^5-a	130 ± 17^5-a
DMPS/5	3645 ± 792^5-a	112 ± 35^5-a
DMPS/10	3765 ± 785^5-a	117 ± 33^5-a
DMPS/60	4009 ± 617^5-a	178 ± 70^5-a
Experiment 3
Buffer/1	3282 ± 430	1035 ± 158
Buffer/5	2602 ± 371	1785 ± 148
Buffer/10	2306 ± 307	2045 ± 208
Buffer/60	2125 ± 353	2122 ± 266
GSH/1	3430 ± 362	205 ± 30^5-a
GSH/5	3005 ± 567	244 ± 16^5-a
GSH/10	3455 ± 406^5-a	280 ± 33^5-a
GSH/60	3284 ± 546^5-a	391 ± 49^5-a

Hg accumulation in renal PT cells was measured as described in the legend to table 1 except that final Hg and thiol concentrations were 5 μM and 20 μM, respectively. Results are the mean ± S.E. of measurements from four or five separate cell preparations.

↵5-a Significantly different (P < .05) from corresponding value in paired samples incubated with Hg alone (“buffer”).