Table 3

Total extracellular and cellular Hg content in renal PT cells incubated with 1 μM Hg: Effect of extracellular thiols

Incubation/time	Extracellular Hg	Cellular Hg
min	pmol
Experiment 1
Buffer/1	525 ± 94	168 ± 72
Buffer/5	495 ± 104	233 ± 96
Buffer/10	459 ± 112	290 ± 117
Buffer/60	414 ± 107	352 ± 119
BSA/1	776 ± 70^3-a	35.8 ± 7.9^3-a
BSA/5	746 ± 90^3-a	40.1 ± 7.4^3-a
BSA/10	699 ± 93^3-a	44.7 ± 10.7^3-a
BSA/60	680 ± 109^3-a	68.0 ± 16.5^3-a
Experiment 2
Buffer/1	393 ± 102	408 ± 103
Buffer/5	301 ± 56	614 ± 122
Buffer/10	282 ± 34	691 ± 105
Buffer/60	240 ± 28	744 ± 78
DMPS/1	833 ± 58^3-a	35.9 ± 8.4^3-a
DMPS/5	806 ± 14^3-a	40.5 ± 12.0^3-a
DMPS/10	740 ± 42^3-a	54.0 ± 22.8^3-a
DMPS/60	721 ± 48^3-a	69.7 ± 18.8^3-a
Experiment 3
Buffer/1	519 ± 27	376 ± 70
Buffer/5	418 ± 15	531 ± 45
Buffer/10	405 ± 13	566 ± 42
Buffer/60	379 ± 9	622 ± 42
GSH/1	703 ± 67^3-a	69.0 ± 5.9^3-a
GSH/5	686 ± 75^3-a	107 ± 15^3-a
GSH/10	705 ± 47^3-a	125 ± 21^3-a
GSH/60	602 ± 72^3-a	269 ± 61^3-a
Experiment 4
Buffer/1	556 ± 38	602 ± 61
Buffer/5	421 ± 37	804 ± 89
Buffer/10	412 ± 42	837 ± 77
Buffer/60	350 ± 47	943 ± 85
Cys/1	810 ± 80^3-a	189 ± 37^3-a
Cys/5	768 ± 71^3-a	277 ± 43^3-a
Cys/10	718 ± 51^3-a	392 ± 78^3-a
Cys/60	627 ± 63^3-a	616 ± 87^3-a

Hg accumulation in renal PT cells was measured as described in the legend to table 1 except that final Hg and thiol concentrations were 1 and 4 μM, respectively. Results are the mean ± S.E. of measurements from four or five separate cell preparations.

↵3-a Significantly different (P < .05) from corresponding value in paired samples incubated with Hg alone (“buffer”).