Antagonist | 1 μM Carbachol | Nerve-evoked Contractions | ||
---|---|---|---|---|
Longitudinal | Circular | On-contractions | Off-contractions | |
Methoctramine (n) | 5.13 ± 0.04 (11) | 5.08 ± 0.07 (10) | 5.89 ± 0.17 (8) | 5.3 ± 0.15 (6) |
AF-DX-116 (n) | 5.64 ± 0.16 (5) | 5.69 ± 0.12 (7) | 6.90 ± 0.19 (3) | 5.42 ± 0.10 (3) |
p-F-HHSiD | 6.30 ± 0.11 (6) | 6.34 ± 0.08 (4) | 7.56 ± 0.12 (8) | 6.70 ± 0.11 (5) |
Pirenzepine (n) | 5.93 ± 0.06 (10) | 6.23 ± 0.06 (7) | 7.75 ± 0.10 (8) | 6.70 ± 0.13 (4) |
Zamifenacin (n) | 6.76 ± 0.17 (5) | 6.64 ± 0.10 (10) | 8.61 ± 0.05 (4) | 7.80 ± 0.13 (4) |
4-DAMP (n) | 8.13 ± 0.06 (9) | 8.36 ± 0.09 (7) | 10.52 ± 0.09 (8) | 9.1 ± 0.11 (3) |
Negative log IC50 values for six antagonists were determined for contraction to a single concentration of carbachol (1 μM) in muscle strips from the longitudinal and circular muscle strips, and electrical field stimulation (EFS)-evoked, nerve-mediatedon-contractions (100 μM NG-nitro-l-arginine present in the bath) andoff-contractions in circular muscle strips. IC50values were determined as detailed in “Methods.” These data are derived from the experiments shown in figures 1 and 5. Graphical correlations of the IC50 values are illustrated in figures 1C and 5C.