Table 2

Effect of losartan, atropine and hexamethonium given i.c.v. or directly into the PVN before i.c.v. 0.15 M saline on plasma AVP levels

Experimental Protocol	Drug	Site of Drug Application	Plasma AVP Level (pg/ml)
	Basal	—	1.8 ± 0.1
1	0.15 M Saline	i.c.v.	2.3 ± 0.4
2	a) Losartan	i.c.v.
	0.15 M Saline	i.c.v.	3.0 ± 0.6
3	a) Atropine	i.c.v.
	0.15 M Saline	i.c.v.	1.8 ± 0.1
	b) Hexamethonium	i.c.v.
	0.15 M Saline	i.c.v.	2.4 ± 0.2
4	a) Losartan	PVN
	0.15 M Saline	i.c.v.	2.1 ± 0.2
5	a) Atropine	PVN
	0.15 M Saline	i.c.v.	2.0 ± 0.3
	b) Hexamethonium	PVN
	0.15 M Saline	i.c.v.	2.3 ± 0.2

Doses used in i.c.v. experiments: losartan: 10 nmol in 1 μl; atropine or hexamethonium: 100 nmol in 1 μl. Doses used for direct microinjections into the PVN: losartan: 4 nmol in 200 nl; atropine or hexamethonium: 200 nmol in 200 nl.