Role of ATP hydrolysis in mitochondrial GSH uptake in the presence and absence of antimycin A
| Addition | Initial Rate of GSH Uptake (nmol/min per mg Protein) | |
|---|---|---|
| − Antimycin A | + Antimycin A | |
| Buffer | 1.19 ± 0.18 | 1.24 ± 0.14 |
| + 2 mM ATP | 1.06 ± 0.09 | 1.16 ± 0.20 |
| + ATP + oligomycin (20 μg/mL) | 0.12 ± 0.015-a | 0.23 ± 0.085-a |
| + ATP + atractyloside (20 μM) | 1.01 ± 0.12 | 1.05 ± 0.14 |
Time courses of uptake of 5 mM GSH were determined with addition of the indicated compound(s) to the incubation medium in the absence or presence of 2 μM antimycin A. Mitochondrial samples were processed by the centrifugation-resuspension method. The initial rate of GSH uptake was then calculated by the method described in “Materials and Methods.” Results are the means ± S.E. of three experiments.
↵5-a Significant difference (P < .05) from the corresponding control value.