Regional [3H]epibatidine binding at six cytisine concentrations
| Brain Area | Cytisine Concentration (nM) | |||||
|---|---|---|---|---|---|---|
| 0 | 5 | 50 | 150 | 500 | 5000 | |
| Telencephalon | ||||||
| Olfactory bulbs | ||||||
| Glomerular layer | 13.3 ± 1.0 | 15.1 ± 0.7 | 0.0 | 0.0 | 0.0 | 0.0 |
| Internal plexiform layer | 37.4 ± 4.1 | 34.9 ± 1.3 | 0.0 | 0.0 | 0.0 | 0.0 |
| Internal granular layer | 16.9 ± 1.9 | 15.1 ± 1.2 | 0.0 | 0.0 | 0.0 | 0.0 |
| Accessory olfactory bulb | 561.1 ± 46.3 | 529.2 ± 7.9 | 511.1 ± 38.2 | 418.1 ± 75.7 | 249.3 ± 20.9 | 0.0 |
| A.O.B. granular cell | 294.5 ± 16.8 | 258.3 ± 27.8 | 202.1 ± 20.0 | 168.4 ± 25.6 | 109.4 ± 20.3 | 0.0 |
| Anterior olfactory n. | 37.7 ± 7.8 | 32.8 ± 1.6 | 5.2 ± 6.4 | 0.0 | 0.0 | 0.0 |
| Neocortex | ||||||
| Frontal cortex | 64.5 ± 4.1 | 46.5 ± 1.3 | 8.3 ± 5.0 | 7.4 ± 4.8 | 0.0 | 0.0 |
| Cerebral cortex, outer | 42.1 ± 3.1 | 27.5 ± 1.7 | 7.6 ± 1.9 | 0.0 | 0.0 | 0.0 |
| Cerebral cortex, inner | 72.5 ± 2.0 | 51.0 ± 1.5 | 11.8 ± 2.1 | 0.0 | 0.0 | 0.0 |
| Olfactory tubercles | 69.8 ± 2.8 | 54.0 ± 4.7 | 19.0 ± 3.7 | 16.9 ± 8.3 | 0.0 | 0.0 |
| Retrosplenial cortex | 153.8 ± 6.3 | 99.3 ± 2.7 | 30.8 ± 7.1 | 0.0 | 0.0 | 0.0 |
| Basal ganglia | ||||||
| Nucleus accumbens | 64.0 ± 2.0 | 46.6 ± 1.3 | 19.2 ± 1.8 | 14.2 ± 4.0 | 0.0 | 0.0 |
| Caudate putamen | 70.3 ± 0.3 | 49.7 ± 2.9 | 21.3 ± 4.8 | 17.3 ± 5.4 | 0.0 | 0.0 |
| Globus pallidus | 34.4 ± 2.5 | 21.6 ± 3.2 | 6.0 ± 2.5 | 0.9 ± 2.7 | 0.0 | 0.0 |
| Substantia nigra, compacta | 132.6 ± 33.5 | 87.8 ± 21.1 | 23.1 ± 4.8 | 0.0 | 0.0 | 0.0 |
| Substantia nigra, reticulata | 32.4 ± 5.1 | 19.1 ± 5.0 | 3.1 ± 3.0 | 0.0 | 0.0 | 0.0 |
| Ventral tegmental area | 123.3 ± 18.2 | 87.9 ± 15.3 | 25.0 ± 12.5 | 0.0 | 0.0 | 0.0 |
| Hippocampus | ||||||
| Dentate Gyrus | 48.9 ± 2.8 | 34.0 ± 0.7 | 3.9 ± 3.9 | 0.0 | 0.0 | 0.0 |
| Molecular Layer | 16.2 ± 5.0 | 12.6 ± 6.1 | 0.1 ± 0.1 | 0.0 | 0.0 | 0.0 |
| Subiculum | 111.3 ± 3.1 | 68.8 ± 1.8 | 22.4 ± 4.6 | 0.0 | 0.0 | 0.0 |
| Septum | 31.8 ± 4.2 | 19.7 ± 2.5 | 4.9 ± 1.4 | 0.8 ± 4.7 | 0.0 | 0.0 |
| Diencephalon | ||||||
| Thalamus | ||||||
| Paraventricular n. | 109.7 ± 5.7 | 62.2 ± 8.7 | 13.0 ± 2.2 | 6.7 ± 3.8 | 0.0 | 0.0 |
| Anteroventral n. | 122.1 ± 6.3 | 74.9 ± 3.7 | 20.1 ± 3.7 | 18.5 ± 9.5 | 0.0 | 0.0 |
| Anterodorsal n. | 352.7 ± 22.6 | 259.0 ± 27.3 | 58.7 ± 7.9 | 28.6 ± 5.2 | 0.0 | 0.0 |
| Lateral Posterior n. | 268.0 ± 8.7 | 157.3 ± 6.3 | 37.0 ± 2.6 | 24.8 ± 5.2 | 0.0 | 0.0 |
| Ventral Posterior n. | 145.6 ± 13.9 | 93.3 ± 15.2 | 27.4 ± 3.3 | 17.1 ± 6.5 | 0.0 | 0.0 |
| Mediodorsal n. | 186.1 ± 13.4 | 118.5 ± 3.3 | 34.8 ± 4.4 | 8.3 ± 8.3 | 0.0 | 0.0 |
| Hypothalamus | 36.7 ± 7.2 | 24.7 ± 4.5 | 5.6 ± 2.5 | 2.9 ± 3.2 | 0.0 | 0.0 |
| Epithalamus and subthalamus | ||||||
| Medial habenula | 1602 ± 152 | 1617 ± 85 | 1408 ± 122 | 1233 ± 190 | 846 ± 204 | 213 ± 51 |
| Zona incerta | 80.3 ± 2.1 | 55.5 ± 1.7 | 18.9 ± 3.1 | 15.5 ± 7.7 | 0.0 | 0.0 |
| Metathalamus | ||||||
| Dorsal lateral geniculate n. | 428.5 ± 32.6 | 282.4 ± 20.2 | 108.5 ± 7.2 | 71.1 ± 8.1 | 34.0 ± 9.4 | 0.0 |
| Ventral lateral geniculate n. | 348.6 ± 51.2 | 245.8 ± 40.2 | 103.0 ± 26.0 | 74.9 ± 12.5 | 33.2 ± 12.0 | 0.0 |
| Medial geniculate n. | 164.8 ± 6.1 | 115.4 ± 6.4 | 40.7 ± 10.6 | 38.8 ± 10.9 | 0.0 | 0.0 |
| Mesencephalon | ||||||
| Interpeduncular n. | 1473 ± 58 | 1456 ± 159 | 1294 ± 165 | 1175 ± 165 | 767 ± 199 | 89 ± 1 |
| Superior colliculus | ||||||
| Superficial gray | 366.6 ± 8.0 | 325.0 ± 29.2 | 195.5 ± 28.4 | 127.5 ± 7.9 | 75.2 ± 13.3 | 0.0 |
| Deeper layers | 140.3 ± 11.7 | 93.3 ± 2.4 | 44.6 ± 9.1 | 27.6 ± 4.8 | 16.9 ± 8.7 | 0.0 |
| Inferior colliculus | ||||||
| External cortex | 104.4 ± 7.0 | 107.6 ± 3.3 | 69.6 ± 16.5 | 69.5 ± 11.4 | 43.5 ± 16.7 | 0.0 |
| Core | 30.9 ± 4.8 | 25.9 ± 4.2 | 17.9 ± 2.1 | 11.7 ± 3.8 | 5.5 ± 2.6 | 0.0 |
| Central gray | 81.2 ± 4.9 | 49.2 ± 4.0 | 14.2 ± 2.2 | 0.0 | 0.0 | 0.0 |
| Pons | ||||||
| Ventral tegmental n. | 134.3 ± 15.1 | 92.6 ± 2.7 | 55.3 ± 24.8 | 24.6 ± 5.3 | 23.1 ± 12.4 | 0.0 |
| Remaining pons | 41.8 ± 7.8 | 27.9 ± 2.8 | 10.0 ± 5.6 | 9.8 ± 4.5 | 0.0 | 0.0 |
| Cerebellum | 4.4 ± 2.9 | 4.4 ± 1.3 | 2.8 ± 0.8 | 1.9 ± 2.4 | 0.0 | 0.0 |
| Pineal gland | 520.8 ± 31.2 | 670.8 ± 63.2 | 763.9 ± 134.5 | 503.9 ± 46.0 | 236.8 ± 49.7 | 0.0 |
| Fiber tracts | ||||||
| Anterior commissure | 8.9 ± 4.6 | 10.0 ± 1.7 | −0.4 ± 0.6 | 0.2 ± 2.4 | 0.0 | 0.0 |
| Corpus callosum | 6.4 ± 2.6 | 5.8 ± 2.4 | −2.4 ± 1.3 | −1.3 ± 0.4 | 0.0 | 0.0 |
| Fasciculus retroflexus | 519.4 ± 39.3 | 511.0 ± 39.3 | 453.5 ± 38.5 | 323.4 ± 25.9 | 177.6 ± 28.5 | 0.0 |
Brain sections (14-μ thick) were incubated with 400 nM [3H]epibatidine and one of the indicated concentrations of cytisine. After a 3-wk exposure to βmax3H film, signal intensity was quantitated as described in “Materials and Methods” and specific binding was calculated by comparison to3H-containing standards. Values are presented in fmol of epibatidine bound per mg of tissue. Each value represents the mean ± S.E.M. from three different mice. Six individual measurements of signal intensity were made on each region from each mouse when possible. Entries of 0.0 correspond to areas that could no longer be identified and/or did not differ from film background.