Times | [35S]GTPγS binding | [3H]Diprenorphine binding | ||
---|---|---|---|---|
EC50 | Maximal binding | Kd | Bmax | |
nM | fmol/mg protein | nM | fmol/mg protein | |
Control | 3.8 ± 0.5 | 191 ± 3 | 0.16 ± 0.02 | 1217 ± 48 |
15 min | 5.4 ± 0.7 | 144 ± 71-a | 0.16 ± 0.01 | 1373 ± 43 |
1 h | 8.5 ± 0.7 | 118 ± 91-a | 0.14 ± 0.01 | 1253 ± 18 |
4 h | 10.4 ± 1.41-a | 101 ± 51-a | 0.15 ± 0.01 | 901 ± 341-a |
24 h | 23.5 ± 7.41-a | 89 ± 61-a | 0.15 ± 0.01 | 992 ± 321-a |
CHO-hkor cells were treated with 1 μM (−)U50,488H for various times and washed, and the (−)U50,488H-induced increases in [35S]GTPγS binding and [3H]diprenorphine binding to the receptor were performed as described under “Materials and Methods.” Each datum represents mean ± S.E.M. of three to six experiments. Data were analyzed by analysis of variance followed by Sheffe F-test.
↵1-a P < .05 compared with the control.