Agonist pharmacology of hGluR3i determined by [Ca++]i measurements in HEK69-8 cells
| Compound | Potency | Efficacy | n | |||
|---|---|---|---|---|---|---|
| EC50values | nH | Statistics | Maximum response | Statistically different from glutamate? | ||
| μM | ||||||
| Quisqualate | 1.49 (1.29, 1.74) | 1.8 ± 0.3 | g,a,k,d,f | 95 ± 7 | No | 4 |
| Domoate | 4.59 (3.1, 6.80) | 1.4 ± 0.2 | g,a,q | 45 ± 10 | Yes | 3 |
| Kainate | 8.05 (6.74, 9.6) | 1.9 ± 0.3 | g,q | 50 ± 4 | Yes | 7 |
| Fluorowillardiine | 10.8 (9.1, 12.8) | 1.6 ± 0.2 | q | 84 ± 5 | No | 3 |
| AMPA | 11.0 (9.3, 13.0) | 1.4 ± 0.1 | d,q | 102 ± 3 | No | 9 |
| Glutamate | 21.9 (19.4, 24.7) | 1.6 ± 0.2 | k,d,q | 100 | – | 9 |
EC50 values, determined from [Ca++]imeasurements performed in HEK69-8 cells, are expressed as the geometric mean (lower, upper S.E.), and the Hill slope, nH, expressed as the mean ± S.E. The efficacy is expressed relative to the response elicited by 1 mM glutamate, run in the same experiment, and represents the mean ± S.E. Statistical comparison of potencies was performed with ANOVA followed by Student-Newman-Keuls pairwise comparison. Differences (P < .05) are indicated by the letters: g, a, k, d, q and f, where values are different from glutamate, AMPA, kainate, domoate, quisqualate and fluorowillardiine, respectively. Statistical comparison of the efficacies of agonists relative to glutamate was performed with ANOVA followed by Dunnett’s test, with differences (P < .05) indicated appropriately.