Specific cpm6-a | |||
---|---|---|---|
125I-huTNFα (6 × 10−10 M) | + ITF1779 (50 μM) | + ITF1493 (50 μM) | |
Medium | 1486 | 3182 (+114%)6-b | 1525 (+2%) |
+ AcD | 580 | 1874 (+223%) | 500 (−14%) |
+ CHX | 128 | 526 (+310%) | 130 (+1%) |
LM cells were cultured overnight in 24-well plates. Then medium was removed, and cells were treated for 2 h at 37°C with 2 μg/ml of AcD or CHX. Binding of 125I-huTNFα, in the absence or presence of ITF1779 or ITF1493 or a 100-fold excess of unlabeled huTNFα, was performed for 4 h at 37°C.
↵6-a See note b in table 3.
↵6-b Numbers in brackets are the percent increase (+) or decrease (−) of specific 125I-huTNFα binding.