Composition of internal and external solutions used in this study (mM)
| A | B | C | D | E | F | G | H | |
|---|---|---|---|---|---|---|---|---|
| NaCl | 150 | – | 150 | – | 145 | – | – | 150 |
| KCl | 5.4 | 125 | 5.4 | 125 | 5.4 | 15 | 140 | 5.4 |
| MgCl2 | 1.2 | 4 | 1.2 | 4 | 1.2 | 1 | 1.4 | 1.2 |
| HEPES | 5 | 10 | 5 | 10 | 5 | 10 | 10 | 5 |
| EGTA | – | 0.02 | 2.5 | 6 | – | – | – | – |
| CaCl2 | 1.2 | – | – | 3.25 | 1.2 | – | 1 | – |
| Aspartic acid | – | – | – | – | – | 125 | – | – |
| KOH | – | – | – | – | – | 125 | – | – |
| Glucose | 10 | – | 10 | – | 10 | – | – | 10 |
| ATP-Na2 | – | – | – | 5 | – | – | 5 | – |
| ATP-Mg | – | – | – | – | – | – | 0.25 | – |
| GTP-Na | – | – | – | 0.5 | – | – | 0.5 | – |
| pH | 7.4 | 7.3 | 7.4 | 7.3 | 7.4 | 7.3 | 7.2 | 7.4 |
| NaOH | KOH | NaOH | KOH | NaOH | KOH | KOH | NaOH |