Potency of monoamine uptake inhibitors to displace [3H]nisoxetine (2.0 nM) binding to GD 20 rat placental membranes
| Drug | IC50 | Ki | Hill coefficient |
|---|---|---|---|
| nM | |||
| Desipramine | 2.66 ± 0.29 | 0.95 ± 0.12 | 0.99 ± 0.05 |
| Nisoxetine | 3.75 ± 0.32 | 1.32 ± 0.12 | 1.02 ± 0.02 |
| RTI-55 | 21.6 ± 0.06 | 7.6 ± 0.24 | 0.96 ± 0.07 |
| Nomifensine | 28.9 ± 1.12 | 10.2 ± 0.42 | 0.94 ± 0.04 |
| Difluoropine | 1333 ± 368 | 458 ± 118 | 1.16 ± 0.05 |
| Cocaine | 2823 ± 128 | 1008 ± 39 | 0.98 ± 0.06 |
| Citalopram | 8203 ± 119 | 2930 ± 127 | 1.03 ± 0.03 |
| Bupropion | 30,693 ± 1004 | 10,900 ± 212 | 0.97 ± 0.13 |
| Zimelidine | 35,217 ± 125 | 12,290 ± 203 | 1.18 ± 0.01 |
| GBR 12909 | 66,953 ± 19,437 | 23,923 ± 7206 | 0.91 ± 0.06 |
Membrane fractions were prepared as described in Materials and Methods. Two different drugs were tested in each experiment, using a tissue pool obtained from four placentas each from two different dams. Each value represents the mean ± S.E.M. of three separate experiments for each drug.