Anti-HSV-1 activity of fractions prepared from G. japonicum
| Fractions | EC50 | CC50 |
|---|---|---|
| μg/ml | ||
| EtOAc-extractable fraction | 51.4 ± 5.3* | – |
| Sephadex LH-20 column chromatography | ||
| Fraction 50 | 16.5 | – |
| Fraction 51 | 17.5 | – |
| HPLC | ||
| Fraction P1 | >20 | – |
| Fraction P2 | >20 | – |
| Fraction P3 | >20 | – |
| Fraction P4 (eugeniin) | 5.0 ± 0.61 | 69.3 ± 9.4** |
| (66***,77*** *) | ||
| Fraction P5 | 16.5 | – |
| Fraction P6 | >20 | – |
The herbal extract of G. japonicum was fractionated by EtOAc extraction, Sephadex LH-20 column chromatography and HPLC. The fractions separated in each step were examined for anti-HSV activity in the plaque-reduction assay. The cytotoxicity of fraction P4 (eugeniin) was determined by the growth inhibition or [3H]thymidine uptake assay as described in the text. EC50 and CC50values were determined graphically. An asterisk (*) indicates a mean value ± S.D. of three independent experiments. Two asterisks (**) indicate the CC50 value determined by the growth inhibition assay with Vero cells. Three asterisks (***) indicate the CC50value determined by the [3H]thymidine uptake assay with Vero cells. Four asterisks (****) indicate the CC50 value determined by the growth inhibition of HEL cells.