Table 6

Voltage-dependent modulation of Ca++ currents by NPY receptors

GroupInhibition6-a
HEK 293 G1A1HEK 293 E52
Without prepulseWith prepulseWithout prepulseWith prepulse
Y155.5  ± 9.129.1  ± 13.38.80  ± 4.874.75  ± 0.56
Y265.7  ± 8.833.1  ± 12.019.50  ± 10.4212.80  ± 3.31
Y444.0  ± 9.917.5  ± 1.8016.73  ± 9.19.4  ± 4.40

Data show the inhibition of Ca++ currents by NPY analogs before and after a depolarizing prepulse to +80 mV (fig. 4). Recordings of Ca++ currents were made from HEK 293 cells stably expressing N- or R-type Ca++ channels transfected with cDNAs for Y1, Y2 or Y4 receptors. Each value represents the mean ± S.E.M. from three to four experiments. The agonists used were NPY (100 nM) for Y1, PYY (100 nM) for Y2 and hPP (100 nM) for Y4 receptors.

  • ↵6-a The values, means ± S.E.M., are percentages.