Effects of EGTA on anti-IgE-induced contractions in human airways in absence or presence of AIC
| Response to ACh (g) | Effects of Drugs on Basal Tone (g) | Emax anti-IgE (1/1000) (%ACh) | |
|---|---|---|---|
| Control | 2.33 ± 0.42 | −0.04 ± 0.03 | 63 ± 13 |
| EGTA | 2.04 ± 0.20 | −0.30 ± 0.05* | 26 ± 13* |
| AIC | 1.86 ± 0.32 | −0.47 ± 0.09 | 99 ± 19 |
| AIC and EGTA | 1.88 ± 0.41 | −0.45 ± 0.10 | 41 ± 12* |
Human bronchial rings were equilibrated for 90 min and stimulated with ACh (100 μM). After washing, the preparations were incubated 5 min in Tyrode’s solution or in Tyrode’s solution containing EGTA (4 mM). Tissues were then stimulated with a-IgE (dilution 1:1000). Protocols were performed in untreated tissues or in tissues pretreated with AIC (30 min).
Values are means ± S.E.M. obtained from seven lung samples.
* Values different from appropriate control data (P < .05).