Metabolism of linoleic acid by recombinant human CYP and by adult and fetal human liver microsomes to epoxides/diols, 11-HODE, 17-HODE (ω2) and 18-HODE (ω1)
| CYP | Incubation time | Rate | Percent of total metabolites | |||
|---|---|---|---|---|---|---|
| ∑Epoxides/diols | 11-HODE | ω2 | ω1 | |||
| min | pmol/min/pmol | % | ||||
| CYP1A21-a | 120 | 0.06 | 41 | 17 | 22 | 14 |
| CYP2C8 | 30 | 0.3 | 50 | 8 | 5 | 14 |
| CYP2C9 | 30 | 0.6 | 62 | 5 | 2 | 18 |
| CYP2C19 | 30 | 0.2 | 54 | 4 | 23 | 11 |
| CYP3A4 | 30 | 0.4 | 37 | 28 | 6 | 13 |
| Liver microsomes | Rate | |||||
| nmol/min/mg | ||||||
| HL37 | 0.18 | 35 | 8 | 7 | 32 | |
| HL42 | 0.19 | 31 | 6 | 6 | 41 | |
| HL46 | 0.19 | 30 | 6 | 6 | 37 | |
| FL2 | 0.11 | 33 | 19 | 6 | 24 | |
↵1-a CYP1A2 was expressed in lymphoblastoid cells, whereas the others were expressed in insect cells together with NADPH-P450 reductase. The CYP3A4 was also expressed with cytochromeb5. In all experiment, unidentified polar metabolites were also formed (<20% of total metabolites), and some of these were also present in control cells without expressed CYP. Liver microsomes were incubated for 30 min.