Effect of increasing concentrations of the MEK inhibitor PD098059 on the potency and maximal effect of 5-HT in the endothelium denuded rat aorta
| PD098059 concentration | Potency −log EC50 | Maximum contraction |
|---|---|---|
| μM | M | % PE contraction |
| Vehicle (4) | 6.17 ± 0.09 | 108.15 ± 6.27 |
| 1 (3) | 6.40 ± 0.13 | 100.83 ± 6.82 |
| 3 (6) | 5.78 ± 0.061-a | 85.63 ± 2.911-a |
| 10 (4) | 5.56 ± 0.141-a | 77.86 ± 6.811-a |
| 30 (4) | 5.33 ± 0.051-a | 67.35 ± 11.771-a |
| 50 (4) | 5.35 ± 0.051-a | 86.65 ± 6.551-a |
| 100 (4) | 5.53 ± 0.041-a | 89.00 ± 8.441-a |
Data are presented as mean ± S.E.M. for the number of animals in parentheses. The EC50 value was defined as the concentration of 5-HT necessary to produce a half-maximal response (determined as described in Methods). Maximum contraction is reported as a percentage of contraction in response to phenylephrine (10−5 M).
↵1-a Statistically significant differences between vehicle (greatest percentage of DMSO, 0.2%) and PD098059 responses (P < .05, ANOVA followed by Tukey’s test).