Effect of CEC and (+)-niguldipine on [3H]prazosin binding in rat arteries before and after chemical sympathectomy3-a
| Intact Rats | Sympathectomized Rats | |||||
|---|---|---|---|---|---|---|
| Control | CEC | (+)-Niguldipine | Control | CEC | (+)-Niguldipine | |
| fmol/mg protein | fmol/mg protein | |||||
| Thoracic aorta | 24.3 ± 2.5 | 0.80 ± 0.6* | 18.5 ± 3.0 | 23.2 ± 6.5 | 1.9 ± 0.8* | 23.1 ± 5.6 |
| Carotid artery | 20.3 ± 4.7 | 0.05 ± 1.9* | 24.3 ± 1.8 | 30.0 ± 6.8 | 2.03 ± 1.6* | 32.9 ± 4.3 |
| Mammary artery | 13.0 ± 5.0 | 1.1 ± 2.2* | 6.4 ± 1.6* | N.A. | N.A. | N.A. |
| Renal artery | 15.5 ± 3.5 | 2.8 ± 2.3* | 8.1 ± 0.5* | N.A. | N.A | N.A |
| Femoral artery | 12.4 ± 1.9 | 1.6 ± 1.8* | 7.6 ± 1.1* | 9.2 ± 2.2† | 1.2 ± 0.9* | 15.0 ± 6.3 |
| Mesenteric arteries | ||||||
| Superior | 25.5 ± 2.2 | 5.2 ± 1.5* | 24.3 ± 1.7 | 15.0 ± 1.6† | 1.1 ± 0.9* | 16.4 ± 6.9 |
| Small (first order branch) | 46.8 ± 2.5 | 27.9 ± 7.1* | 10.9 ± 5.2* | 28.1 ± 7.6† | 6.6 ± 3.2* | 18.1 ± 4.2* |
| Resistance-sized (third order branch) | 28.4 ± 4.8 | 20.0 ± 4.8 | 6.8 ± 2.4* | 45.3 ± 14.7 | 8.6 ± 3.6* | 20.4 ± 5.3* |
↵3-a Specific binding observed in the presence of 300 pM [3H]prazosin in control conditions, after pretreatment with CEC (100 μM, 30 min) or in the presence of (+)-niguldipine (30 nM) is shown for arteries of intact rats and of rats that had been chemically sympathectomized with 6-hydroxydopamine. Mean ± S.E.M. (n = 12). N.A., not available.
†,* the difference is statistically significant (P < .05) from intact rats or control values, respectively.