Phycoerythrin Fluorescence Intensity | ||
---|---|---|
R1EGO Cells | C57BL/6ByJ Thymocytes | |
a.u. | a.u. | |
FITC-AA | 314 ± 97 | 5.5 ± 0.6 |
FITC-AA/nor-BNI | 65 ± 29 | 2.4 ± 0.3 |
% Specific fluorescence | 83 ± 5 | 55 ± 4 |
Kappa opioid receptor labeling was carried out as described under “Materials and Methods.” Autofluorescence control intensities were 4.3 ± 0.2 arbitrary units (a.u.) for R1EGO cells and 1.6 ± 0.1 a.u. for gated (R1) thymocytes. Background staining (biotinylated antifluorescein IgG/PE in the absence of FITC-AA) was 124 ± 26 a.u. for R1EGO cells and 2.9 ± 0.1 a.u. for thymocytes. Data represent the mean ± S.E.M. of relative median peak fluorescence intensity values from three to seven experiments performed in triplicate, with the background fluorescence subtracted. Percent specific fluorescence was calculated by relative median fluorescence values as: