Inhibition of specific [3H]prazosin binding to membranes prepared from control and diabetic rat aorta and caudal artery, assayed in the presence of 200 mM NaCl
| Artery | Animal | −Gpp(NH)p | +Gpp(NH)p | ||||
|---|---|---|---|---|---|---|---|
| IC50 of high-affinity site | IC50 of lowaffinity site | Fraction of high-affinity sites (%) | Fraction of low-affinity sites (%) | IC50 of lowaffinity site | Fraction of low-affinity sites (%) | ||
| Aorta | Control | N.D. | 5.60 ± 0.18 | 0 | 100 | 5.49 ± 0.31 | 100 |
| (n = 5) | (0.58 μM) | (0.75 μM) | |||||
| Diabetic | N.D. | 5.59 ± 0.14 | 0 | 100 | 5.36 ± 0.22 | 100 | |
| (n = 6) | (0.61 μM) | (1.04 μM) | |||||
| Caudal | Control | N.D. | 5.15 ± 0.07 | 0 | 100 | 4.86 ± 0.074-a | 100 |
| (n = 6) | (2.43 μM) | (4.84 μM) | |||||
| Diabetic | N.D. | 5.08 ± 0.03 | 0 | 100 | 4.77 ± 0.094-a | 100 | |
| (n = 6) | (2.54 μM) | (5.19 μM) | |||||
Estimates of Ki values are shown in parentheses.
↵4-a Significantly (P < .05) different from corresponding affinity obtained in the absence of Gpp(NH)p.
N.D. = not detected.