Time of lysis | Concentration of PD 166285 exposed to cells in culture | |||
---|---|---|---|---|
0.01 μM | 0.1 μM | 1.0 μM | 10 μM | |
Concentrations of PD 166285 in cell lysates | ||||
ng/ml | ||||
0 hr | <253-a | 71.2 | 710 | 11,821 |
24 hr | <25 | <25 | 167 | 4,807 |
48 hr | <25 | <25 | 71.2 | 2,330 |
72 hr | <25 | <25 | 42.3 | 1,433 |
96 hr | <25 | <25 | 23.4 | 461 |
↵3-a The limit of detection for PD 166285 viahigh-performance liquid chromatography/UV detection was 25 ng/ml.