Lack of effect of ARL 15896AR in the two-vessel occlusion/hypotension model of global ischemia (12 min): evaluation of rat CA1 hippocampal and striatal neurons
| Experimental | Hippocampal CA1 Neurons | Striatal Neurons | ||
|---|---|---|---|---|
| Vehicle | ARL 15896AR | Vehicle | ARL 15896AR | |
| Pentobarbital | ||||
| Anesthesia—I1-a | ||||
| ARL 15896AR | 2.7 ± 0.91 | 3.0 ± 0 | ||
| (500 ng/ml) | (n = 10) | (n = 3) | ||
| ARL 15896AR | 2.5 ± 0.95 | |||
| (1000 ng/ml) | (n = 12) | |||
| Pentobarbital | ||||
| Anesthesia—II1-b | ||||
| ARL 15896AR | 61.1% ± 10 | 69.5% ± 8 | 70 ± 251-c | 92 ± 32 |
| (12 mg/kg, b.i.d./ | (n = 6) | (n = 7) | (n = 6) | (n = 7) |
| 7 days, s.c.) | ||||
| Nitrous oxide/ | ||||
| halothane anesthesia1-b | ||||
| ARL 15896AR | 53.7% ± 18 | 45% ± 11 | 84 ± 231-c | 52 ± 15 |
| (24 mg/kg, b.i.d./ | (n = 5) | (n = 9) | (n = 5) | (n = 9) |
| 7 days, p.o.) | ||||
Values are the combined means ± S.E. of all levels of sections including left and right sides of the brain.
↵1-a In the pentobarbital anesthesia–I study hippocampal CA1 neuronal damage is quantified using a gross scoring technique rating bilaterally 6 sections taken along the mid-septotemporal hippocampus: 3 = 67–100%, 2 = 34–66%, 1 = <33% and 0 = no damage.
↵1-b In the pentobarbital anesthesia–II study and with the nitrous oxide/halothane anesthesia study, the hippocampus is examined at six levels along the septotemporal axis. Damage at each level is expressed as a percentage of the total CA1 setter (see “Methods”).
↵1-c Striatal damage is quantified by examination of two 630-μm diameter microscopic fields in the dorsolateral caudate at the coronal level of septal nuclei (+0.2 mm from Bregma). Values represent total number of acidophilic neurons in the two fields.