Treatment Paradigm | Δ [Ca++]i | |
---|---|---|
Control | Treated | |
nM | nM | |
7-Day CEE | 193.1 ± 18.1 (26) | 148.6 ± 11.1* (32) |
1-Day CEE | 145.8 ± 11.5 (35) | 179.3 ± 13.1 (17) |
2-Day CEE | 112.6 ± 14.6 (24) | 118.2 ± 15.7 (20) |
Chronic APV | 122.0 ± 14.5 (12) | 160.8 ± 16.6 (12) |
Chronic ethanol + APV | 122.0 ± 14.5 (12) | 93.4 ± 23.2 (7) |
KA-induced calcium responses were not consistently altered across treatment paradigms (see “Methods”). Values represent the mean ± S.E.M. of increases in [Ca++]i (Δ [Ca++]i = peak agonist-stimulated [Ca++]i minus basal [Ca++]i) induced by 25 μM KA in control and treated neurons. Values in parentheses are number of neurons from two to five separate sister culture preparations. Statistical significance determined by two-tailedt test (*P < .05).