Drugs | Uptake Clearance | |
---|---|---|
LLC-pBK | LLC-OAT-K1 | |
μl/mg protein/5 min | ||
[3H]Methotrexate | 1.6 ± 0.0 | 3.0 ± 0.2*** |
[14C]Indomethacin | 11.2 ± 0.3 | 9.7 ± 0.7 |
[3H]Methotrexate (100 nM, 28.5 kBq/ml) and [14C]indomethacin (35 μM, 5.7 kBq/ml) accumulations from the basolateral side (1 ml, pH 7.4) were measured for 5 min at 37°C. Unlabeled incubation medium without drugs was added to the apical side (0.5 ml, pH 7.4). The accumulations are expressed as uptake clearance.
↵2-a Each value represents the mean ± S.E. of three monolayers. *** P < .001, significant difference from LLC-pBK.