Effect of ANF on contractility, cGMP levels and PKG activity ratios in spontaneously-contracting rat proximal and distal colon
| n | cGMP (pmol/g tissue) | −cGMP / +cGMP (pmol PO4/min/mg protein) | PKG Activity Ratio | Inhibition of Spontaneous Contractions? | |
|---|---|---|---|---|---|
| Distal Colon | |||||
| Control | 7 | 0.42 ± 0.04 | 0.91 ± 0.21 / 11 1 ± 2 | 0.07 ± 0.01 | |
| ANF (100 nM) | 7 | 1.1 ± 0.1* | 1.40 ± 0.17 / 10 ± 1 | 0.14 ± 0.01* | No |
| Proximal Colon | |||||
| Control | 7 | 0.47 ± 0.03 | 0.82 ± 0.13 / 10 ± 1 | 0.08 ± 0.01 | |
| ANF (100 nM) | 7 | 1.5 ± 0.2* | 1.2 ± 0.2 / 7.5 ± 0.9 | 0.18 ± 0.03* | Yes |
Values are mean ± S.E. Means were compared with their controls by using a paired Student’s t test.
* Significantly different from control (P < .05).