Effect of SNP on cGMP levels, PKG activity and contractility in PE-contracted rabbit aorta
| n | cGMP (pmol/g tissue) | −cyclic GMP / +cyclic GMP (pmol PO4/min/mg protein) | PKG Activity Ratio | Relaxation (%) | |
|---|---|---|---|---|---|
| Control | 11 | 0.37 ± 0.06 | 44 ± 6 / 281 ± 56 | 0.16 ± 0.02 | — |
| SNP (0.1 μM) | 11 | 1.0 ± 0.2* | 55 ± 8 / 273 ± 41 | 0.22 ± 0.04* | 18 ± 4* |
| Control | 6 | 0.27 ± 0.04 | 44 ± 3 / 235 ± 31 | 0.19 ± 0.02 | — |
| SNP (1.0 μM) | 6 | 3.7 ± 0.5* | 63 ± 12* / 233 ± 47 | 0.28 ± 0.02* | 58 ± 4* |
| Control | 10 | 0.28 ± 0.03 | 54 ± 8 / 283 ± 34 | 0.19 ± 0.02 | — |
| SNP (10.0 μM) | 10 | 16 ± 4* | 87 ± 13* / 247 ± 31 | 0.36 ± 0.03* | 71 ± 3* |
Values are mean ± S.E.M. SNP-treated strips were exposed to SNP for 2 min. Means were compared with their controls by using a paired Student’s t test. * Significantly different from PE control (P < .05).