Table 2

Inhibition of cAMP accumulation by N/OFQ and peptides

PeptidecAMP Accumulation
IC50 (nM)% Maximal Inhibition
N/OFQ1.46  ± 0.3584  ± 10
1 Ac-RYYRWR-NH2 0.53  ± 0.0475  ± 2
2 Ac-RYYRWK-NH2 0.53  ± 0.2758  ± 32-a
3 Ac-RYYRIK-NH2 1.16  ± 0.7462  ± 52-a
4 Ac-RYYKWR-NH2 1.95  ± 0.968  ± 22-a
5 Ac-RYYKWK-NH2 0.97  ± 0.966  ± 142-a

Experiments were conducted on rat ORL1 transfected into CHO cells. Data shown are the average ± SD of at least three experiments conducted in triplicate.

  • 2-a Statistically significant difference from percent maximal inhibition induced by N/OFQ, P < .05 by analysis of variance with post hoc Newman-Keuls.