Table 1

Initial and fitted parameter values in in vitro experiments

Parameters	Units	Initial Values	Fitted Values
k _on R	μl/hr/mg protein	52.1	60.5 ± 5.77
k _int	hr⁻¹	0.160	0.149 ± 0.046
k _off	hr⁻¹	0.132	0.025 ± 0.0529
k _eff	hr⁻¹	0.448	0.477 ± 0.148

In vitro experimental data shown in figures 1 and 2 were analyzed based on a model shown in scheme 1. Initial parameter values were determined from the plot shown in figure 3. The data were fitted simultaneously to equations 1, 2 and 3 for determining each parameter value.