High Expression Group | Low Expression Group | |||
---|---|---|---|---|
Wild type | Mutant | Wild type | Mutant | |
Number of clones | 2 | 3 | 2 | 2 |
B max (fmol/mg protein) | 754 ± 65 | 891 ± 150 | 364 ± 52 | 258 ± 50 |
K d(nM) | 5 ± 1 | 3 ± 1 | 5 ± 2 | 6 ± 1 |
1-a Results of two to four assays per clone analyzed by the method of Scatchard (1949) to obtain theBmax and equilibriumKd.