Catalytic activities of P450ML2d in the reconstituted system
| Activity (nmol/min/nmol P450) | ||
|---|---|---|
| Debrisoquine 4-hydroxylation | ND2-a | (0.047)2-b |
| Propranolol 4-hydroxylation | 1.39 | (0.857) |
| Propranolol 5-hydroxylation | 0.100 | (0.064) |
| Propranolol 7-hydroxylation | ND 2-a | (0.141) |
| Imipramine 2-hydroxylation | 2.55 | (0.845) |
| Testosterone 2α-hydroxylation | ND 2-a | (ND) |
| Testosterone 6β-hydroxylation | 0.224 | (0.115) |
| Testosterone 7α-hydroxylation | 0.181 | (0.994) |
| Testosterone 16α-hydroxylation | 0.841 | (0.716) |
Each reconstituted system (1 ml) contained P450ML2d (50 pmol), NADPH-P450-reductase (0.5 U), dilauroylphosphatidylcholine (5 nmol), sodium cholate (0.1 mg), NADPH-generating system and debrisoquine (2 mM), propranolol (1 mM), imipramine (100 μM) or testosterone (250 μM).
↵2-a ND, Not detectable.
↵2-b Values in parentheses indicate the activities in liver microsomes of male ddY rats.