Description | Formation oftrans-Oxime5-b | |
---|---|---|
Phenethylamine | Phenethylamine hydroxylamine | |
nmol/min/mg of protein | ||
Complete5-a | 17.40 ± 2.64 | 21.20 ± 0.43 |
+n-Octylamine (5.0 mM) | 17.54 ± 0.165-c | 23.11 ± 2.60 |
+Thiobenzamide (5.0 mM) | 4.03 ± 0.51 (77%) | 12.90 ± 2.51 (40%) |
+Heat inactivation (+10 units catalase) | N.D.5-d (100%) | N.D. (100%) |
↵5-a The complete system was the same as table 1 except it contained 1.2 mM concentration of substrate and 70 μg of human Lys158 FMO3-MBP in potassium phosphate buffer, pH 9.0.
↵5-b Data are expressed as mean ± S.D. of 3 determinations.
↵5-c Percent inhibition is provided in parentheses.
↵5-d N.D., nondetectable. The limit of detection was 50 pmol/min/mg of protein.