Description | trans-Oxime1-b |
---|---|
nmol/min/mg of protein | |
Complete1-a | 3.67 ± 0.66 |
+n-Octylamine (5.0 mM) | 2.58 ± 0.90 (29.7%)1-c |
+Thiourea (5.0 mM) | 2.03 ± 0.17 (44.7%) |
+Thiobenzamide (5.0 mM) | 1.80 ± 0.18 (51.0%) |
+Heat inactivation (+10 units catalase) | 0.94 ± 0.12 (74.4%) |
↵1-a The complete system contained 1.2 mM phenylethylamine1, 0.4 mM NADP+, 0.4 mM glucose-6-phosphate, 1 IU of glucose-6-phosphate dehydrogenase, 1.2 mM DETAPAC and 0.57 mg of adult human liver microsomes in potassium phosphate buffer, pH 8.0. The incubation time was 10 min at 37°C.
↵1-b Data are expressed as mean ± S.D. of 3 determinations. The limit of detection was 50 pmol of oxime/min/mg of protein.
↵1-c Percent inhibition is provided in parentheses.