Table 1

Binding assay conditions

Parameter	5-HT_1A^1-a	5-HT_2A^1-b	5-HT_2C^1-c	D₂^1-a	alpha₁^1-d	D₁^1-e
Ligand	8-OH-DPAT	Ketanserin	Mesulergine	YM-09151-2	Prazosin	SCH 23390
Concentration	0.2 nM	0.2 nM	1 nM	0.05 nM	0.1 nM	0.3 nM
Tissue	Rat cortex	Rat cortex	Pig cortex	Rat striatum	Rat cortex	Rat striatum
Concentration	10 mg/ml	10 mg/ml	40 mg/ml	1 mg/ml	5 mg/ml	0.75 mg/ml
Incubation time	30 min	30 min	60 min	60 min	30 min	30 min
Nonspecific	5-HT	Methysergide	Mianserin	(+)-Butaclamol	Phentolamine	SKF 38393
Concentration	10 μM	10 μM	10 μM	1 μM	50 μM	10 μM

↵1-a Assié et al., 1993.
↵1-b Leysen et al., 1982.
↵1-c Liau et al., 1991.
↵1-d Hornung et al., 1979.
↵1-e Billard et al., 1984.