Cytosolic inducible nitric oxide synthase activity
| Specific Activity (pmol/mg/min) | |
|---|---|
| Control | 1.6 ± 1.2 |
| Control + NMA | 1.9 ± 1.3 |
| Control + 5 mM APAP | 1.5 ± 0.7 |
| IL-1 | 58 ± 135-a |
| IL-1 + NMA | 63 ± 15 |
| IL-1 + 2 mM APAP | 95 ± 115-b |
| IL-1 + 5 mM APAP | 167 ± 275-c |
| IL-1 + 5 mM APAP + NAC (200 μM) | 97 ± 115-b |
| IL-1 + 5 mM APAP + NAC (200 μM) + SOD (500 U/ml) + CAT (300 U/ml) + DFO (20 μM) + BHT (100 μM) | 66 ± 95-d |
Control cells were incubated in media alone. Hepatocytes in primary culture were otherwise treated for a period of 18 hr as indicated. Cytosolic iNOS enzyme activity was determined as described in “Materials and Methods.” Data are expressed as mean ± S.E.M. of four experiments. Statistical analysis was performed with the Student’s t test. NMA, N-monomethyl-l-arginine; NAC, N-acetylcysteine; APAP, acetaminophen; IL-1, interleukin-1; SOD, superoxide dismutase; CAT, catalase; DFO, desferrioxamine; BHT, butylated hydroxytoluene.
↵5-a P < .01 vs. control.
↵5-b P < .03 vs. control, IL-1, IL-1 + NMA, or IL-1 + 5 mM APAP.
↵5-c P < .01 vs. control, IL-1 or IL-1 + NMA.
↵5-d P < .01 vs. control, IL-1 + 2 mM APAP, or IL-1 + 5 mM APAP.