Table 1

Effects of transport inhibitors on NBD-rapamycin accumulation in killifish proximal tubules

Treatment	n	Fluorescence Intensity
Treatment	n	Cell	Lumen
Control (0.5 μM)	12	25 ± 3	79 ± 8
1 μM rapamycin	7	31 ± 3	29 ± 4^**
5 μM rapamycin	9	34 ± 7	15 ± 3^**
5 μM CSA	11	25 ± 4	14 ± 2^**
Control (1.0 μM)	9	16 ± 2	50 ± 5
1 mM PAH	5	19 ± 3	53 ± 6
1 mM TEA	7	17 ± 1	52 ± 2
0.5 μM LTC₄	6	14 ± 2	47 ± 5
100 μM Verapamil	7	18 ± 3	11 ± 1^**
Control (1.0 μM)	7	10 ± 1	31 ± 4
0.1 μM FK506	7	10 ± 1	16 ± 3^*
1.0 μM FK506	7	12 ± 2	8 ± 2^**
Control (1.0 μM)	8	11 ± 1	37 ± 6
0.1 μM PSC-833	9	13 ± 1	25 ± 3
0.5 μM PSC-833	7	12 ± 2	16 ± 2^**
1.0 μM PSC-833	7	15 ± 2	11 ± 1^**

Tubules were incubated in medium containing NBD-rapamycin without (control) and with the indicated chemicals. The substrate concentration used for each experiment is given in parentheses. After 60 min, confocal images were acquired and analyzed as described under “Materials and Methods.” Data are given as mean ± S.E.;n is the number of tubules. The variability in fluorescence intensities for controls reflect primarily differences in photomultiplier gain settings, although some animal-to-animal variation was observed. Statistical comparisons: ^* significantly lower than controls, P < .05; ^** significantly lower than controls, P < .01.