Pretreatment4-a | Challenge | Response |
---|---|---|
Body temperature4-a | Δt°C ± S.E. | |
Saline | Saline | −0.3 ± 0.1 |
Saline | Nicotine (1.5) | −4.1 ± 0.3 |
Saline | Lobeline (5) | −3.4 ± 0.2 |
Nicotine (4) | Nicotine (1.5) | −1.2 ± 0.14-b |
Nicotine (4) | Lobeline (5) | −3.4 ± 0.2 |
Tail-flick test4-c | % MPE ± S.E. | |
---|---|---|
Saline | Saline | 5 ± 2 |
Saline | Nicotine (20) | 75 ± 17 |
Saline | Lobeline (40) | 75 ± 18 |
Nicotine (1) | Nicotine (40) | 7 ± 24-d |
Nicotine (1) | Lobeline (40) | 82 ± 12 |
Nicotine (5) | Lobeline (40) | 90 ± 10 |
Nicotine (20) | Lobeline (40) | 100 ± 0 |
↵4-a Doses are expressed in mg/kg in parentheses—mice were challenged with either s.c. nicotine or lobeline 3 hr after nicotine pretreatment at 4 mg/kg.
↵4-b Statistically different from saline-nicotine 1.5 at P < .05.
↵4-c Doses are expressed in μg/animal—mice were challenged with either i.t. nicotine or lobeline 10 min after nicotine pretreatment at 1 μg/animal.
↵4-d Statistically different from saline-nicotine 20 at P < .05.