Used for | Human Liver | R/S Ratio | S-Mephenytoin 4′-Hydroxylation | Desipramine 2-Hydroxylation | |||||
---|---|---|---|---|---|---|---|---|---|
Km (μM) | Vmax (pmol/mg/min) | Vmax/Km (μl/mg/min) | Putative Phenotype | Km (μM) | Vmax (pmol/mg/min) | Vmax/Km (μl/mg/min) | |||
Kinetic1-a and inhibition studies | HL -32 | 0.75 | 25.9 | 20 | 0.78 | PM | 3.28 | 282 | 85.8 |
Kinetic1-a and inhibition studies | HL -33 | 0.10 | 18.2 | 98 | 5.36 | EM | 6.94 | 89 | 12.8 |
Kinetic1-a and inhibition studies | HL -34 | 0.06 | 5.5 | 305 | 19.69 | EM | 3.27 | 240 | 73.4 |
Kinetic1-a study | HL -35 | 0.83 | 74.8 | 12 | 0.16 | PM | 9.97 | 53 | 5.3 |
Kinetic1-a and inhibition studies | HL -36 | 0.06 | 22.0 | 217 | 9.87 | EM | 4.69 | 484 | 103.1 |
Kinetic1-a and inhibition studies | HL -37 | 0.40 | 29.1 | 15 | 0.42 | PM | 8.48 | 102 | 12.0 |
R/S was determined as the ratio of the formation rate of 4′-hydroxymephenytoin with 1 mM of R-mephenytoin to that with 1 mM of S-mephenytoin. Microsomal samples with a ratio >0.7 were classified as those derived from the putative PM patients. However, HL-37 was identified as a putative PM liver by its lowerV max andV max/K m values, although the R/S value obtained from this liver was <0.7.
↵1-a Kinetic study for Eadie-Hoffstee plots.