Potency of opioids to increase Gα-like immunoreactivity in mouse PAG membranes
| Opioid | Gi2α | Gx/zα | ||
|---|---|---|---|---|
| ED501-a | Slope1-b | ED50 | Slope | |
| Morphine | 0.80 ± 0.06 nM | 11.76 ± 1.93 | 0.17 ± 0.01 pM | 18.86 ± 3.20 |
| β-Endorphin | 4.5 ± 0.2 nM | 12.65 ± 1.60 | 0.60 ± 0.07 pM | 15.87 ± 2.51 |
| DAMGO | 16.0 ± 1.4 pM | 12.20 ± 1.93 | 0.36 ± 0.05 nM | 14.49 ± 2.31 |
| DPDPE | ∼6 pM1-c | 5.98 ± 2.15 | Inactive | |
| [d-Ala2]deltorphin II | 4.20 ± 0.39 fM | 24.39 ± 6.54 | 0.30 ± 0.07 pM1-d | 6.49 ± 1.64 |
↵1-a The apparent ED50s (mean ± S.E.M.) are calculated from three independent determinations.
↵1-b Slopes and 95% confidence intervals were calculated using the SigmaStat computer program.
↵1-c The maximum effect was 40% the activation produced by [d-Ala2]deltorphin II on this Gα-like ir.
↵1-d The maximum effect was 50% the activation promoted bymu receptor-binding agonists on Gα-like ir.