Mediuml-Glutamine (mM) | l-Arginine Concentrations in Medium (mM) | ||
---|---|---|---|
0.1 | 0.4 | 1 | |
nmol/5 min/106 cells | |||
0 | 0.91 ± 0.08c | 3.48 ± 0.42b | 7.46 ± 0.81a |
0.5 | 0.87 ± 0.09c | 3.26 ± 0.44b | 7.73 ± 0.92a |
2 | 0.93 ± 0.11c | 3.57 ± 0.48b | 7.85 ± 0.96a |
Cultured bovine venular endothelial cells were washed three times with 10 ml of KHB buffer and then incubated in 0.2 ml of KHB buffer (pH 7.4) at 37°C for 5 min. The incubation medium contained 0.1, 0.4 and 1.0 mM l-[U-14C]arginine (2 × 106dpm/0.2 ml). Data are mean ± S.E.M., n = 5, and are analyzed by two-way ANOVA.
a-c P < .05: means that have different superscripts within a row are different.