Table 4

Uptake of l-arginine by bovine venular endothelial cells

Mediuml-Glutamine (mM)l-Arginine Concentrations in Medium (mM)
0.10.41
nmol/5 min/106 cells
00.91  ± 0.08c 3.48  ± 0.42b 7.46  ± 0.81a
0.50.87  ± 0.09c 3.26  ± 0.44b 7.73  ± 0.92a
20.93  ± 0.11c 3.57  ± 0.48b 7.85  ± 0.96a

Cultured bovine venular endothelial cells were washed three times with 10 ml of KHB buffer and then incubated in 0.2 ml of KHB buffer (pH 7.4) at 37°C for 5 min. The incubation medium contained 0.1, 0.4 and 1.0 mM l-[U-14C]arginine (2 × 106dpm/0.2 ml). Data are mean ± S.E.M., n = 5, and are analyzed by two-way ANOVA.

  • a-c P < .05: means that have different superscripts within a row are different.