Inhibitor | 10 min | 20 min |
---|---|---|
Phloridzin | ||
10 mM | 76.5 ± 1.5* | 73.5 ± 3.0* |
1 mM | 93.6 ± 2.2 | 93.7 ± 4.8 |
Propentofylline | ||
10 mM | 61.9 ± 2.5* | 60.4 ± 1.9* |
1 mM | 81.5 ± 1.4* | 78.8 ± 2.8* |
Dilazep | ||
100 μM | 91.9 ± 3.2 | 88.6 ± 2.9* |
Dipyridamole | ||
100 μM1-a | 76.6 ± 4.8* | 78.2 ± 2.8* |
10 μM1-b | 91.6 ± 0.8 | 91.3 ± 5.1 |
NBMPR | ||
100 μM1-a | 74.9 ± 3.6* | 80.2 ± 1.8* |
10 μM1-b | 89.3 ± 0.9* | 103.1 ± 5.4 |
Cells were loaded with [3H]formycin B, extracellular tritium was removed and cells were resuspended in choline buffer (37°C) in the absence or presence of test compounds. After 10- or 20-min release intervals, the tritium content of supernatants was determined. [3H]Formycin B released in the presence of inhibitors is expressed as a percent of control release, determined in the absence of added compounds. Experiments consisted of controls and two drug concentrations, and they were performed in quadruplicate and repeated at least three times.
* P < .05 ANOVA with Tukey’s HSD post test; relative to control values
↵1-a Dissolved in 1% DMSO; [3H]formycin B released in 1% DMSO was 99.1 ± 1.7% and 100.8 ± 2.0% of control at 10 and 20 min, respectively.
↵1-b Dissolved 0.1% DMSO; [3H]formycin B released in 0.1% DMSO was 97.8 ± 1.9% and 99.8 ± 1.1% of control at 10 min and 20 min, respectively.