Effect of cromakalim with or without glyburide on postischemic cardiac function and coronary flow in isolated rat hearts
| Predrug | Reperfusion 30 min | |
|---|---|---|
| HR (bpm) | ||
| Vehicle | 290 ± 13 | 251 ± 124-a |
| 10 μM cromakalim (pre/post) | 295 ± 10 | 298 ± 74-b |
| 10 μM cromakalim (reper only) | 288 ± 5 | 259 ± 114-a |
| 1 μM glyburide (pre) | 298 ± 11 | 250 ± 74-a |
| 10 μM CRO (pre/post) + 1 μM GLY (pre/post) | 291 ± 7 | 221 ± 324-a |
| 10 μM CRO (pre/post) + 1 μM GLY (reper only) | 286 ± 14 | 301 ± 5 |
| LVDP (mm Hg) | ||
| Vehicle | 122 ± 4 | 16 ± 64-a |
| 10 μM cromakalim (pre/post) | 122 ± 9 | 61 ± 6a,b |
| 10 μM cromakalim (reper only) | 123 ± 4 | 14 ± 64-a |
| 1 μM glyburide (pre) | 119 ± 15 | 14 ± 24-a |
| 10 μM CRO (pre/post) + 1 μM GLY (pre/post) | 130 ± 3 | 11 ± 54-a |
| 10 μM CRO (pre/post) + 1 μM GLY (reper only) | 126 ± 7 | 60 ± 4a,b |
| Coronary flow (ml/min/g) | ||
| Vehicle | 18.9 ± 0.6 | 15.4 ± 1.24-a |
| 10 μM cromakalim (pre/post) | 19.7 ± 1.5 | 23.9 ± 1.1a,b |
| 10 μM cromakalim (reper only) | 18.3 ± 2.6 | 17.5 ± 2.0 |
| 1 μM glyburide (pre) | 17.9 ± 1.8 | 14.1 ± 1.9 |
| 10 μM CRO (pre/post) + 1 μM GLY (pre/post) | 19.8 ± 1.1 | 11.8 ± 2.24-a |
| 10 μM CRO (pre/post) + 1 μM GLY (reper only) | 20.1 ± 2.1 | 22.3 ± 1.84-b |
All values are mean ± S.E.M.; pre/post, treatment both before and after ischemia; reper only, treatment only during reperfusion; pre, treatment before ischemia; CRO, cromakalim; GLY, glyburide.
↵4-a Significantly different from its respective predrug value (P < .05).
↵4-b Significantly different from its respective vehicle group value (P < .05).