Effects of glutamate agonists on APP release from cortical and hippocampal slices
| Glutamate agonist | APP release | |
|---|---|---|
| Cortical slices | Hippocampal slices | |
| % of first 10-min period | ||
| l-Glutamic acid (μM) | ||
| 0 | 93 ± 4 (12) | 93 ± 3 (11) |
| 5 | 100 ± 6 (4) | 116 ± 10 (6) |
| 50 | 102 ± 8 (7) | 114 ± 6 (6) |
| 500 | 139 ± 9 (13)2-a | 124 ± 7 (11)2-a |
| trans-(1S,3R)-ACPD (μM) | ||
| 0 | 88 ± 6 (9) | 93 ± 6 (9) |
| 1 | 152 ± 17 (6)2-a | 128 ± 20 (8) |
| 10 | 188 ± 13 (8)2-a | 137 ± 18 (9)2-a |
| 100 | 163 ± 16 (7)2-a | 159 ± 17 (9)2-a |
| Quisqualic acid (μM) | ||
| 0 | 83 ± 4 (11) | 91 ± 5 (9) |
| 1 | 108 ± 5 (11)2-a | 127 ± 7 (9)2-a |
| 10 | 114 ± 5 (11)2-a | 125 ± 6 (9)2-a |
| 100 | 118 ± 6 (11)2-a | 114 ± 9 (9) |
| Kainic acid (μM) | ||
| 0 | 92 ± 6 (9) | 89 ± 4 (8) |
| 5 | 97 ± 7 (9) | 114 ± 6 (8) |
| 50 | 95 ± 8 (9) | 97 ± 8 (8) |
| 500 | 109 ± 8 (9) | 100 ± 8 (8) |
| AMPA (μM) | ||
| 0 | 86 ± 9 (9) | 92 ± 4 (8) |
| 1 | 96 ± 7 (9) | 109 ± 7 (8) |
| 10 | 89 ± 6 (9) | 100 ± 6 (8) |
| 100 | 92 ± 7 (9) | 107 ± 7 (8) |
| NMDA (μM) | ||
| 0 | 99 ± 6 (8) | 97 ± 7 (8) |
| 10 | 115 ± 15 (9) | 94 ± 7 (8) |
| 100 | 100 ± 12 (9) | 98 ± 9 (8) |
| 320 | 111 ± 14 (8) | 102 ± 6 (8) |
Cortical and hippocampal slices were perfused for 60 min (0.8 ml/min) and incubated for 20 min with Krebs’ medium. After an 80-min equilibration period, slices were incubated in 1 ml of Krebs’ medium for two consecutive 10-min periods. During the second 10-min incubation period, the incubation medium contained the concentrations of glutamate agonist indicated. Incubation media were removed at the end of each 10-min incubation period, and the slices were rinsed with 0.5 ml of Krebs’ medium and assayed as described in table 1. Data are mean ± S.E.M. The number of measurements is given in parentheses.
↵2-a Significantly higher than the corresponding control (0) value.