Effect of TPGS on the level of ROS, protein-bound sulfhydryl groups and TBA-RS in hepatocytes cultured for 20 h with CsA2-a
| CsA | TPGS | ROS | Protein SH | TBA-RS |
|---|---|---|---|---|
| μM | μM | F/mg, % control | nmol/mg | nmol/ml |
| 0 | 0 | 100 ± 9 | 115.3 ± 10.8 | 0.01 ± 0.01 |
| (n = 3) | (n = 7) | (n = 3) | ||
| 250 | 118 ± 14 | 114.4 ± 6.1 | 0.09 | |
| (n = 3) | (n = 3) | |||
| 25 | 0 | 168 ± 19* | 100.0 ± 17.0** | 0.20 ± 0.01* |
| (n = 3) | (n = 3) | (n = 2) | ||
| 250 | 161 ± 3 | 120.3 ± 4.4# | 0.08 | |
| (n = 3) | (n = 3) | |||
| 50 | 0 | 210 ± 34** | 73.8 ± 22.3*** | 0.22 ± 0.09* |
| (n = 3) | (n = 7) | (n = 3) | ||
| 250 | 151 ± 4# | 118.5 ± 10.1# | 0.03 | |
| (n = 3) | (n = 3) |
↵2-a Values (mean ± S.D.) refer either to milligrams of cellular protein or to milliliters of cell culture medium. Protein-SH were determined in cells cultured with 2 mM BSO. TBA-RS were determined in the cell culture supernatant. Data were statistically significantly different than the respective 0 μM TPGS group. # p < .05; versus the respective 0 μM CsA group, * p < .05, ** p < .01, *** p < .001. F = fluorescence intensity.