Comparison of the apparent brain uptake of [3H]-3-O-methyl-d-glucose, [14C]-mannitol, [14C]-butanol and [3H]-water determined by carotid artery injection with and without 10% DMSO in the injection solution
| Substrate | Control | +10% DMSO | ||
|---|---|---|---|---|
| % dose per g brain | BUI (%) | % dose per g brain | BUI (%) | |
| [3H]-3OMG | 1.64 ± 0.31 | 13.9 ± 1.2 | 1.35 ± 0.23 | 11.6 ± 0.8 |
| [14C]-butanol | 11.6 ± 1.1 | 11.6 ± 0.8 | ||
| [14C]-mannitol | 0.190 ± 0.038 | 3.36 ± 0.56 | 0.205 ± 0.024 | 2.77 ± 0.30 |
| [3H]-water | 6.47 ± 1.36 | 7.47 ± 0.54 | ||
The apparent first-pass extraction of each substrate was determined for 15 sec after carotid artery injection, with or without 10% DMSO in the injectate. Each value represents the mean ± S.E. of five experiments. No significant difference was observed for the percentage dose per g brain in the presence and absence of DMSO in any of the experiments.