Effect of NAD or NADPH on acetaldehyde metabolism in rat liver homogenates
| Homogenate | Acetate Formation | NADPH/NAD Ratio | |
|---|---|---|---|
| NAD Additive | NADPH Additive | ||
| nmol/min/mg protein | % | ||
| Control | 5.99 ± 1.47 | 0.72 ± 0.38 | 12.22 ± 6.90 |
| Ethanol-treated | 4.76 ± 1.76 | 1.60 ± 0.70* | 33.61 ± 7.81* |
The reaction mixture contained microsomes (200 μg), 300 μM acetaldehyde and 10 mM NADPH or 1.7 mM NAD+ in a final volume of 100 μl, which was incubated for 15 min at 37°C in 0.1 M Tris-acetate buffer, pH 7.2. Results are expressed as nanomoles per minute per milligram of protein and are from five experiments in duplicate (mean values ± S.D.). The NADPH/NAD ratios are expressed as percent.
* Significantly different from control, P < .05.