Table 3

Effect of flow rate on 4MU metabolite formation in rat liver of the serially perfused I+L preparation3-a

Perfusate Flow Rate (Q)
8 ml/min10 ml/min
Flow rate to liver (ml/min/g)1.1  ± 0.141.4  ± 0.2
4MU input rate to intestine, Q C SMA(dpm/min)1,943,000  ± 642,0002,423,000  ± 737,000
(Total) hepatic venous outflow rate:QC HV (% 4MU input rate to the intestine)
 4MUS43  ± 349  ± 7*
 4MUG39  ± 538  ± 3
 4MU2.3  ± 0.52.6  ± 0.6
 Sum: 4MUG + 4MUS + 4MU85  ± 589  ± 7*
Biliary excretion rate (% 4MU input rate to the intestine)
 4MUS9.8  ± 6.17.5  ± 6.2
 4MUG4.4  ± 1.53.0  ± 1.6*
 4MU0.2  ± 0.110.11  ± 0.07*
 Sum: 4MUS + 4MUG + 4MU14  ± 611  ± 7*
Summed hepatic venous outflow rate and biliary excretion rate (% 4MU input rate to the intestine)
 4MUS53  ± 556  ± 4
 4MUG44  ± 541  ± 4
 4MU2.5  ± 0.42.7  ± 0.6
 Sum: 4MUS + 4MUG + 4MU99  ± 0.9100  ± 0.6
Hepatic metabolite formation rate3-b (% 4MU input rate to the intestine)
 4MUS35  ± 939  ± 9*
 4MUG5  ± 39  ± 3*
 Sum: 4MUS + 4MUG40  ± 748  ± 6*

  • ↵3-a Steady-state data were expressed as mean ± S.D. of six experiments. (Livers weights were 7.3 ± 0.9 g.) Data were expressed as percent of rate to the intestine.

  • ↵3-b Equation 10. (Data were expressed as percent of rate to the intestine.)

  • * Data were significantly different from those at 8 ml/min; P < .05.